CCC instrument operation requires fluidic control to;
- Select the direction of mobile phase flow through the column
- Select the column to be used
- Interface with test and measurement equipment
Sample injection is unique compared with other forms of chromatography. Samples being dissolved in either phase of the solvent system or sometimes as a mixtures of the two.
Injection volumes can be as large as 10 - 15% of the column volume. In DE equipment injection is typically through a sample loop which is combined into one valve box. (Manual and automated options).
Valve Box Comparison
|HPCCC Column Selection
(Normal Phase/Reverse Phase)
||Manual fill –
|Manual fill –
|Maximum Number Samples
|HPCCC Instrument Compatibility